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Search for "cell lines" in Full Text gives 110 result(s) in Beilstein Journal of Nanotechnology.
Exploring the relationships between physiochemical properties of nanoparticles and cell damage to combat cancer growth using simple periodic table-based descriptors
Beilstein J. Nanotechnol. 2024, 15, 297–309, doi:10.3762/bjnano.15.27
- structural diversity of metal oxide nanoparticles (MeOx NPs) poses significant challenges in determining their toxic effect on living cells [14][15]. Works related to nanoscale toxicity modeling have been published [16][17][18][19][20] to predict the toxicity profile of MeOx NPs on various cell lines and
Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics
Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17
- additional 10 min. Cell culture The cell lines HepG2, MCF-7, 3T3, and Hek were cultured in DMEM, 10% FBS, and 1% antibiotic at 37 °C and 5% CO2. Cell uptake estimation The cells were seeded onto 6-well plates at 100,000 cells/well and were cultured overnight. On the next day, the cells were incubated with
Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study
Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93
- cytotoxicity of CUR-HSA-MPs showed promising anticancer potential against human hepatocellular carcinoma (Huh-7) and human breast adenocarcinoma (MCF-7) cell lines, although this effect was less pronounced in human dermal fibroblasts (HDFB) and human cholangiocyte (MMN) cell lines. Confocal microscopy was used
- network, which decreases and prolongs drug release [30]. In vitro interaction of HSA-MPs and CUR-HSA-MPs with cells Cytotoxicity toward tumor cells Cytotoxicity of CUR-HSA-MPs in Huh-7 and MCF-7 cancer cell lines was measured using MTT assay. Notably, HSA-MPs revealed no significant cell death among Huh-7
- hepatocellular carcinoma (Huh-7) and human breast adenocarcinoma (MCF-7) cell lines using the colorimetric MTT assay. Human dermal fibroblasts (HDFB) and human cholangiocyte (MMNK-1) cell lines were evaluated as controls for cytotoxicity. Briefly, the cell lines were cultured in cell culture medium containing
Quercetin- and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles: one-pot synthesis, characterization, and anticancer and antibacterial activities
Beilstein J. Nanotechnol. 2023, 14, 362–376, doi:10.3762/bjnano.14.31
- glioblastoma) and ARPE-19 (human retinal pigment epithelium) cells. Both NPs showed anticancer activity, but Ch/Q-Ag NPs seemed to be more effective on cancer cell lines (U-118 MG) in comparison to healthy ones (ARPE-19). Furthermore, the antibacterial activity of Ch/Q- and Ch/CA-Ag NPs against Gram-negative
- high dose (500 µM) for 24 h. It decreased cell viability by 75% in glioblastoma cells and by 25% in non-cancerous cells (data not shown). From this, it can be concluded that the selected cancer drug is highly specific to the cancer cells [56]. Therefore, human glioblastoma (U-118 MG) cell lines were
- cell lines (MCF-7 and HepG2) [57][58]. Materials and Methods Chemicals Chitosan (medium molecular weight: 190,000–310,000 Da based on viscosity; 75–85% deacetylated; viscosity: 200–800 cP, that is 1 wt % in 1% acetic acid at 25 °C), sodium borohydride (NaBH4, ≥98.0%), caffeic acid (≥98.0%), and
Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer
Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23
- activity, including the downstream TKI resistance mutations (Figure 1) [70]. Lu et al. tested the efficacy of combined siRNA treatment with gefitinib in several NSCLC cell lines (A549, H1975, and H1993). The A549 cell line carried wild-type EGFR and KRAS mutations, H1975 cells expressed L858R/T790M EGFR
- for silencing the expression of resistance-related genes was evidenced by a significant reduction in cell growth and increased rate of apoptosis compared to the cells treated with siRNA only. Furthermore, considering that these cell lines are EGFR-positive TKI-resistant NSCLC cells, a synergistic
Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine
Beilstein J. Nanotechnol. 2023, 14, 165–174, doi:10.3762/bjnano.14.17
- region (Figure 6b). The obtained results indicate that these dots can be used for visualization of different cell lines because of the low cytotoxicity and poor antibacterial activity. Conclusion In this study we discussed for the first time the effect of the precursor o-phenylenediamine on structural
In search of cytotoxic selectivity on cancer cells with biogenically synthesized Ag/AgCl nanoparticles
Beilstein J. Nanotechnol. 2022, 13, 1505–1519, doi:10.3762/bjnano.13.124
- synthesized by green methods and, in some cases, are combined with other metals [2]. AgNPs have potential uses in biomedicine. Several authors have reported the ability of AgNPs to act as antibacterial [3][4] or as cytotoxic agents in certain cancer cell lines [5][6]. This type of application has attracted a
- . This result is consistent with Park et al. [45], who reported that the size of AgNPs is an important factor in cytotoxicity, inflammation, and genotoxicity. In this sense, AgNPs have been shown to induce cytotoxicity through apoptosis and necrosis in different cell lines [46]. Microscopic analysis
Recent advances in green carbon dots (2015–2022): synthesis, metal ion sensing, and biological applications
Beilstein J. Nanotechnol. 2022, 13, 1068–1107, doi:10.3762/bjnano.13.93
Bioselectivity of silk protein-based materials and their bio-inspired applications
Beilstein J. Nanotechnol. 2022, 13, 902–921, doi:10.3762/bjnano.13.81
- –usher polymerization pathway in Gram-negative microorganisms involved in pili protein synthesis [79]. For instance, the inhibitor targets pilus chaperone PapD, thereby reducing the adhesion to cell lines by 90% [80]. These pilicides inhibit curli formation in uropathogenic E. coli by preventing the
Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy
Beilstein J. Nanotechnol. 2022, 13, 560–569, doi:10.3762/bjnano.13.47
- migration capacity, and cells on soft substrates (3 kPa) had the lowest migration capacity (Figure 2c). Cell proliferation assays also revealed that both cell lines had a significantly greater ability to proliferate on stiff substrates, and that the proliferation of prostate cancer cells significantly
- protrusion on the surface of the cell pseudopod. In contrast, the surface of PC-3 cells on soft substrates was smoother. In addition, both cell lines on the stiff substrate were observed to be taller than those on the other two substrates, with cell height values ranging from 1–4 μm. The height of the HPV-PZ
Ethosomal (−)-epigallocatechin-3-gallate as a novel approach to enhance antioxidant, anti-collagenase and anti-elastase effects
Beilstein J. Nanotechnol. 2022, 13, 491–502, doi:10.3762/bjnano.13.41
- dose determined based on cell viability results of 50% or more is appropriate [22][28]. In previous studies, the cytotoxic effect of EGCG, whose antioxidant effect has been proven many times, has been investigated, especially on cancer cell lines. Although there is no cytotoxicity study on the L929
- human cancer cells without causing toxicity problems in normal cells. In general, it has been confirmed that they are relatively nontoxic in healthy L929 cells but toxic in cancer cell lines [29]. Preparation and characterization of formulations Like many phenolic compounds, EGCG is not sufficiently
Engineered titania nanomaterials in advanced clinical applications
Beilstein J. Nanotechnol. 2022, 13, 201–218, doi:10.3762/bjnano.13.15
- titanium (cpTi). Thereafter, human osteoblast-like cell lines (hFOB) were cultured over annealed Ti, PCL, and PCL/TiO2 (2, 5, and 7 wt % TiO2) scaffolds. SEM images of the cell morphologies are shown in Figure 2. The addition of TiO2 nanoparticles enhances the wettability and surface area, thus favoring
Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles
Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6
- partial PEI oxidation during the synthesis. Here, we demonstrate in vitro dye-free optical imaging and successful gene transfection with luminescent SPION@bPEI, which was further modified for receptor-mediated delivery of the cargo selectively to cancer cell lines overexpressing the epidermal growth
- delivery of an anionic cargo. Besides, a strong intracellular optical signal supports the optically traceable nature of these nanoparticles. SPION@bPEI nanoparticles were further conjugated with Erbitux (Erb), which is an anti-EGFR antibody for targeting EGFR-overexpressing cancer cell lines. SPION@bPEI
- as well as its targeted delivery to epidermal growth factor receptor (EGFR)-positive cancer cell lines, in vitro. Initially, the dose dependent cytotoxicity of the nanoparticles was determined. Then, using a fluorescence microscope, the ability of these nanoparticles to generate intracellular optical
Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy
Beilstein J. Nanotechnol. 2021, 12, 1372–1379, doi:10.3762/bjnano.12.101
- . The sharp contrast reduced the details of the cell surface microstructure of HDPE6-C7, AsPC-1, and MIA-PaCa-2 cell lines. The corresponding analyses of cell surface roughness is listed in Supporting Information File 1, Table S1, which shows that the surface roughness of MIA-PaCa-2, HDPE6-C7, and AsPC
- a relatively linear FDC while the other three cancer cell lines form nonlinear FDCs, which can be evaluated regarding the slope of the FDCs [25]. The Young’s modulus of the four kinds of cells obtained by fitting the linear part of the withdrawal curves are 14.93, 2.1, 6.24 and 3.74 kPa, which
- ]. The statistical comparison of the energy dissipation in the four types of cell lines is shown in Figure 4b. The energy dissipation in the normal cell line (HDPE6-C7) is smaller than that in the PCCs (AsPC-1, MIA PaCa-2, and BxPC-3) and the stiffer the cells, the larger the energy dissipation. This
Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles
Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99
- vitro compared to free drug controls. The IC50 values ranged from 0.81 to 3.97 μg/mL for HepG2 and HT144 cells, whereas IC50 values for normal lymphocytes were 10 to 35 times higher (18.35–43.04 µg/mL). Cobalt ferrite (CFO) and zinc ferrite (ZFO) NPs were highly genotoxic (p < 0.05) in cancer cell lines
- spheroid diameter and up to 74 ± 8.9% of cell death after two weeks. In addition, they also inhibited multidrug resistance (MDR) pump activity in both cell lines suggesting effectivity in MDR cancers. Among the tested MFe2O4 NPs, CFO nanocarriers were the most favorable for targeted cancer therapy due to
- of cell viability were plotted for all doses (Figure 4b) and IC50 values were determined (Table 8). In both cell lines, drug-functionalized NPs caused almost 45–50% reduction in cellular viability at a concentration of 1 µg/mL, and the cellular viability decreased even further at higher doses. In
Use of nanosystems to improve the anticancer effects of curcumin
Beilstein J. Nanotechnol. 2021, 12, 1047–1062, doi:10.3762/bjnano.12.78
- stored at 4 °C) has shown inhibitory effects on A549 (IC50 3.9 µg/mL) and on H460 lung cancer (IC50 2.9 µg/mL) cell lines, according to a cell cycle arrest at G2/M [59]. Morevore, the activities of caspase-3, caspase-8, and caspase-9 had a dose-dependent increase in both cell lines in response to both
- , which yielded a dual targeting structure that was able to cross the blood–brain barrier, a significant obstacle when treating brain tumors. Similarly, an improved internalization of CUR-loaded MNP was also observed in HPAF-II and Panc-1 human pancreatic cancer cell lines (54.06% and 53.86%, respectively
- greater potential to produce ROS [143]. A similar behavior was observed in cervical carcinoma cell lines treated with nanoemulsion-photostimulated CUR, where cell viability was reduced to <5% and an increase in caspase-3 and caspase-7 activity was apparent [144]. Inostroza et al. [112] reported the
The role of deep eutectic solvents and carrageenan in synthesizing biocompatible anisotropic metal nanoparticles
Beilstein J. Nanotechnol. 2021, 12, 924–938, doi:10.3762/bjnano.12.69
- cell lines [32]. Carrageenans are sulfated oligosaccharides extracted from red algae. They are composed of galactose and anhydrogalactose sub-units linked through a glycosidic bond. They are mainly categorized into three types depending on the degree of sulfation. The three types are: kappa carrageenan
- osteoblast cytotoxicity tests in cell lines (L02 and L929). Adapted from [116], J. I. González Ocampo et al., “Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite”, J. Biomed. Mater. Res. A., with permission from John Wiley and Sons
Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension
Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62
- properties. Several studies have used different types of cell lines under in vitro conditions to investigate the properties, differential responses, and mechanisms of action of Ag-NPs [10]. Sengstock et al. showed that Ag-NPs attenuate the adipogenic and osteogenic differentiation of human MSCs. These
- technology indicate that telomerase maintains cells in a proliferative state, and its downregulation parallels cellular differentiation [14]. Telomerase is an enzyme responsible for maintaining the telomeric repetition at the end of the chromosomes. Different cell lines have different telomere lengths (TL
A review on nanostructured silver as a basic ingredient in medicine: physicochemical parameters and characterization
Beilstein J. Nanotechnol. 2021, 12, 440–461, doi:10.3762/bjnano.12.36
- ability of AgNPs against infection caused by the respiratory syncytial virus (RSV). Morris et al., by using in vitro and in vivo models in which mice were infected experimentally, concluded that AgNPs effectively reduced RSV replication and pro-inflammatory cytokine production in epithelial cell lines and
The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors
Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31
- -cell-derived factor 1 in discrete, discontinuous areas that influence the homing ability of a variety of tumor cell lines inside the BM, and that these specific molecules determine a microenvironment for early BM tumor metastasis [34]. This specific feature was employed by Mann and co-workers, who
- authors noticed that the cytotoxicity of folate-functionalized liposomes was greater in FR positive cell lines and that the effect could be blocked through the addition of 1 mM folic acid. Additionally, the authors revealed that the functionalized carriers increased the median survival of the mouse
- reflected on the evaluated IC50 values of IM on different cell lines. Additionally, the comparative analysis of the IC50 values on different cell lines revealed that the transferrin receptor expression and the cellular levels of BCR-ABL mRNA affected the efficacy of the formulation. The cell lines with
Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells
Beilstein J. Nanotechnol. 2021, 12, 282–294, doi:10.3762/bjnano.12.23
- cytotoxicity of AgNPs using different cell lines and rodent models [7][21][22][23]. However, some of these cell lines are resistant to toxicity effects that may be induced by AgNPs [24]. Furthermore, there are only a few studies on the bio-uptake and exposure of AgNPs in human PBMCs [25][26][27][28][29][30][31
Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization
Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22
- , albumin-binding proteins are present on various human tumor cell lines derived from solid tumors [59]. In contrast to BSA-SO-MNPs, CavME was the predominant mechanism involved in the uptake of PEG-SO-MNPs. The internalized quantity of PEG-SO-MNPs was substantially lower compared with control cells when F
The nanomorphology of cell surfaces of adhered osteoblasts
Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20
- the only method capable of nanoscopic three-dimensional imaging of living cells without the application of dye labels or other modifications. Though SICM was developed already in 1989 [19], it was not much exploited until the method was used to image a number of murine and human cell lines [23
Imaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy
Beilstein J. Nanotechnol. 2021, 12, 172–179, doi:10.3762/bjnano.12.13
- , while many more cell lines have been reported to be refractory to SARS-CoV-2 infection [15]. Both scanning electron microscopy (SEM) and transmission electron microscopy (TEM) have been used to image SARS-CoV-2 [16][17][18][19][20]. While TEM achieves unsurpassed resolution and can visualize
A review on the green and sustainable synthesis of silver nanoparticles and one-dimensional silver nanostructures
Beilstein J. Nanotechnol. 2021, 12, 102–136, doi:10.3762/bjnano.12.9
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